FastQCFastQC Report
Sun 21 May 2023
EGAF00007938116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422712
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70401.6654365146955847No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35970.8509339692272753No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG33110.7832756108177672No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC26270.6214633130831393No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24430.5779348587217774No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG23830.5637407975169856No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA23060.5455250856375026No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21910.5183198016616515No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG19610.4639092337099491No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC19460.4603607184087511No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19420.459414447661765No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18140.4291337837582089No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC17160.4059501504570488No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14960.35390525937281175No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14620.34586195802342967No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14540.3439694165294574No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG12960.3065917220235054No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12340.29192452544522035No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12280.2905051193247412No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11380.26921402751755336No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT10950.2590416169874524No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10930.2585684816139594No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA10120.2394064989874903No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA10110.2391699313007438No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10040.23751395749351803No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9920.23467514525255967No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9760.23089006226461517No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9410.22261019322848655No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC9380.22190049016824692No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9200.21764227180680937No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9180.21716913643331628No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9110.21551316262609058No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8840.20912583508393423No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8540.20202880448153826No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7860.1859422017827741No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7490.17718919737315242No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG7330.1734041143852079No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC7320.17316754669846135No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7290.17245784363822178No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7280.17222127595147524No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7190.17009216677075645No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC7170.1696190313972634No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7110.1681996252767842No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6810.16110259467438823No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6810.16110259467438823No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6640.1570809439996972No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6460.15282272563825963No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6310.14927421033706165No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6300.1490376426503151No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC6250.14785480421658245No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6020.14241374742141222No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5920.1400480705539469No Hit
TCCGTTTCTGAACCGAGCGAGTCCACCGGTC5880.13910179980696077No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5850.13839209674672118No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA5390.1275099831563807No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5350.1265637124093946No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG4830.11426219269857492No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4780.11307935426484227No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4610.10905770359015121No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4560.10787486515641855No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG4520.10692859440943243No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4500.10645545903593936No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC4450.1052726206022067No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC4440.10503605291546016No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4430.10479948522871364No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC4410.10432634985522059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)