FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007938131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394437
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG24400.618603224342544No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT21810.5529400132340526No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG20470.5189675410775358No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT19880.5040095122922038No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC18070.4581213222897446No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC18060.4578677963781288No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18050.45761427046651304No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG15670.39727510350195344No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14880.3772465564843055No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG13040.3305977887469989No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12920.3275554778076093No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG12160.3082875085248088No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11910.30194936073441386No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT11250.2852166505677713No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10860.27532914001475517No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG10810.27406151045667626No Hit
AACCGTGGATTCCGCCTTCAGGCGGCCACGGGTCATAACACCCG10130.25682174846680206No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT10060.25504706708549146No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG9750.24718776382540178No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9310.23603262371430672No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT9300.23577909780269093No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8990.22791979454260125No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8760.22208869857543792No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG8640.21904638763604833No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG8580.21752523216635355No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8150.20662361796687428No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG8070.20459541067394793No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7890.2000319442648636No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7720.19572200376739504No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7700.19521495194416344No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA7690.19496142603254765No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG7360.18659507094922637No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA7320.1855809673027632No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7210.18279218227498942No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG6560.16631299801996263No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6540.16580594619673106No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA6420.16276363525734147No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6100.154650806085636No Hit
GCGCAGTGGCGGGGAGCCGTCGTTTTCGGAGGACGGCGGCGGAC6000.15211554696947802No Hit
TTCGGACGGTTTTTTGGCAGCACCGGTGCCACCGGTTTCACGAA5830.14780560647200947No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5620.14248156232807774No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5610.14222803641646195No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5430.13766457000737758No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5400.1369039922725302No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5360.13588988862606702No Hit
GTAAGCCAGTACCGCGCGACGGTTTTTGGAGTATGCCGCTTCGT5350.13563636271445123No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5000.12676295580789837No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG4990.12650942989628255No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC4960.12574885216143514No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4940.12524180033820356No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4810.1219459634871982No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG4740.12017128210588762No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC4740.12017128210588762No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4640.11763602298972967No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT4600.11662191934326649No Hit
GTCCAGGTGCATGGTGTCACGGATTTTCTGGTACACGGCAGAAG4460.11307255658064533No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4440.11256550475741373No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG4430.11231197884579794No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4390.11129787519933475No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC4390.11129787519933475No Hit
GTATGCCAGTACCGCACGACGGTTTTTGGAGTAGGCAGCTTCAT4370.11079082337610315No Hit
CTGCACGGTAGCTACCATCGGGACCAGGTTGCGGGCCAGAATGC4340.11003024564125576No Hit
GATGTCACCCAGTTCGGTCAGCACATCTTTTTCCGGGTTTTCGT4320.10952319381802417No Hit
AGATTCCGCCTTCAGACGACCACGGGTCATAACGCCCGCCGTGC4320.10952319381802417No Hit
CTGGTCTGCTTTGGAACGGGTCACCATCGGGTGGGTAGATTTAC4210.10673440879025041No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4150.10521325332055562No Hit
GTTCAGTACATAGCAGCTGCTAGCCCACAGCAGCTGGGATTTGG4090.10369209785086085No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4070.10318504602762925No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC3970.10064978691147128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)