FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007938132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394437
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57721.4633515618463786No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA40281.021202371988429No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23820.6038987214688277No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG23240.5891942185951116No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21960.5567429019082896No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG21390.5422919249461892No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA19520.49488257947403513No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT17950.4550790113503551No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17840.4522902263225813No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16590.4205994873706067No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16400.4157824950499066No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15620.3960074739438745No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14990.38003534151207924No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14710.3729366159868369No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13300.3371894624490096No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13110.33237247012830945No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG12190.30904808625965613No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT11930.30245641255764544No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11520.2920618501813978No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC10600.26873746631274453No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10300.26113168896427064No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC10060.25504706708549146No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9200.23324383868653298No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9090.23045505365875918No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9050.22944095001229603No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8790.22284927631028528No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8660.21955343945927994No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8520.21600407669665878No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8440.2139758694037324No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8060.20434188476233214No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7620.19318674465123709No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7160.18152455271691043No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6680.1693553089593522No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA6560.16631299801996263No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6410.16251010934572568No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG6380.1617495316108783No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6060.1536367024391728No Hit
GAAGATTCCGTCTTCACTTCCACCCGTGCGC5870.14881971011847264No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5630.14273508823969355No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5550.14070688094676717No Hit
GGCGGCGGTGGCGCAAGCGGTGGCGGCGGTG5470.1386786736538408No Hit
GCACTGGGCCAGCGTCGTGCCGATGCAGTTA5420.1374110440957618No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5310.13462225906798805No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5170.1310728963053669No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5110.1295517408356721No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5020.12727000763112994No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5010.12701648171951416No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4920.12473474851497196No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4860.1232135930452772No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4840.12270654122204559No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4830.1224530153104298No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4800.12169243757558242No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC4700.11915717845942445No Hit
TTTCTGCAGACTATGCTGCGTAAAGAGGTTA4660.11814307481296125No Hit
TCTTCCGCTACTGCATGCACCGCAGGTGTTA4650.11788954890134547No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4640.11763602298972967No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4640.11763602298972967No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG4630.11738249707811386No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4550.1153542897851875No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4550.1153542897851875No Hit
GGCACTCTGGATCAGACCAACGGTCTGTTCG4400.11155140111095055No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4390.11129787519933475No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4380.11104434928771896No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4370.11079082337610315No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4360.11053729746448736No Hit
GCGCTGGGCCAGCGTCGCGCTGACGCAGTTA4340.11003024564125576No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4320.10952319381802417No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4250.1077485124367136No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC4180.10597383105540302No Hit
GGCGCACAGGAAGAGCGCGAAGATACTGTAA4160.10546677923217143No Hit
GACGATGTGTGGACCAGCGGTTCTGACTCCG4150.10521325332055562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)