FastQCFastQC Report
Sun 21 May 2023
EGAF00007938134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422364
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA75231.781165061416219No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA37950.8985140779043669No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA34490.8165942173101873No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27510.6513339205045885No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC26660.6312090992603536No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT26540.6283679480258734No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG25290.5987726226667046No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG23560.5578126923696148No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23470.5556818289437546No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC23150.5481054256518074No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG22670.5367408207138866No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC22240.5265600287903325No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18950.448665132445No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC18320.43374908846397897No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13520.3201030390847705No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13520.3201030390847705No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13180.3120531105870765No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12840.30400318208938265No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11230.26588440302677313No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10750.2545197980888523No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG10100.23913022890208446No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9940.23534202725611084No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC9800.2320273508158839No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9260.21924217026072298No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT9200.21782159464348289No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8950.2119025295716491No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8920.21119224176302906No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG8230.19485562216476782No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA8020.18988360750442748No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7340.1737837505090396No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6800.16099856995387865No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6450.15271187885331136No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6410.15176482844181796No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6320.1496339650159578No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC6290.14892367720733776No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6150.14560900076711084No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5800.13732230966654355No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC5750.1361384966521768No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5710.13519144624068338No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5600.13258705760907655No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5580.13211353240332982No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5460.12927238116884962No Hit
AACACCCAGGAACGCATGGAGCTGCGTAAAC5340.1264312299343694No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC5320.1259577047286227No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5190.12287979089126914No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5010.11861806403954882No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG4800.11364604937920847No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA4650.11009461033610818No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC4410.10441230786714777No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4400.10417554526427442No Hit
TCCCACGAGGAACAGCCACGTTATGAAGACC4340.10275496964703432No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4280.1013343940297942No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4230.10015058101542745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)