FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007938135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448049
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCGGTTTCTGGGACGCCAGGGTCCATTCGATCCATGGCT269526.015413492720662No Hit
ACGTACACCGGGTAACGGGAATAGCTTTCTTCCATAACGATGTC167053.7283868505453643No Hit
TGCCTTCTGACGTTGGGCTTCTGCAATCAGGTGGCCCAGGTTGG109212.437456617468179No Hit
AGCCAGGCCTACCAGACCGTATTTTGCACGGAAACGCAGGCCGT91282.0372771728092243No Hit
AATACCGCGGCCCTGGTTGTACACGCCGTGGTGTTCATGTGCGT77641.7328461842343137No Hit
CCATTCGGTGCGACCCAGTGGCTGGGATTCGTAGGAGCTACGGT70231.5674624873618734No Hit
AACCTGCTTACCGTTAGTTTTCATACGGTAGGAGAACCAGCCTG64251.4339949425174479No Hit
TTCATCGAAAACAGAGGCCAGCATGGTATACAGCTCTGGGTTGG60671.35409296751025No Hit
TGCGGTACAAACCAGGACCAGGTACTGTGCATTGTTGAAGCTAC50371.1242073969588147No Hit
GGCACGGCGCAGGCCACGCAGCTGGTACGGGATGTTTTTAGTTT41840.933826434162335No Hit
AGCAGCAAATTCCTGGGCGTCACGGTAATCGCGGTTTTCCATTT32700.7298308890322264No Hit
GCCAAAGGTAACCGGCATACGGTATTTAATGTCGGACTGCGCGT26710.5961401543134791No Hit
TTCGTCCGGCTGCAGCAGACCGAAACGGGTACGCAGATCGTCTT25540.5700269390178306No Hit
AACCAGGATCGGTTCGCCCGGTTCACCATCAGAGCGGGCGGAGC23500.5244962046561872No Hit
CATCTCACCGAACGGTTTCACGGTGCAAACCAGGTAATAATCGT22370.49927574885782583No Hit
ATCAGAGGAATAGATGTGGTAACCAGTTCTTCCAGCGGCGGTTC19750.440800001785519No Hit
GGACATAGCACCGTCGAAAATCAGGGTCGCTTTCTTACCTTTTT14440.3222861785206529No Hit
TTCGTCGATTTCTTCCAGAGAAACCAGGTCGTAGCGACGGTGGG13990.31224263417617265No Hit
GATGCAACGCTGCAGGTTCGGTTGAATCGATATCGCCGCTCATT10960.2446161022566728No Hit
GAAACCCTGGTTGGCAACCGCAGATTCGTAACCCTGATCATAGA10330.23055514017440054No Hit
CTTACCGGAGTCCTTCAGACCACGGATGACAGTGCCAACGCCAC9450.21091443123408377No Hit
ACGTACTTCCCTTGACCATGAATTAGCGCAATCAGCCGTTGTAG8050.1796678488290343No Hit
GTTACGGGTAGACGGGCTCTGGACGTCACCAACGCCGGAAATAC7530.16806197536430167No Hit
ACCCTGGTAACCTGCATCCAGCAGAGAGTTCAGCGGTTCGATCG7350.1640445576265096No Hit
TTCCAGTTTGTCCGCCTGTTCCATCCAAGCACGCAGCATCTCGT6030.1345834942160344No Hit
ACCCTCCAGCGCCAGCAGACCGCCGCCGGTACGGGAGTCCGGCA5600.12498632962019778No Hit
AGACGGAGATTCGTATACGAAAGACTCGTCGCTGACAGTCTGGA5420.12096891188240573No Hit
ACCCTGCGCTACCACCATGTCACCGTGGATCAGTGGTTCGGAAC5050.1127108865324998No Hit
CATACCGTTCATCTGCGGCATACCCATATTGCCCATCTGGTTCA4520.10088182319344535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)