FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007938137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415987
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC40690.97815556736148No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA40640.9769536067232871No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT39860.958203020767476No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA39080.9394524348116648No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA33650.8089195095039028No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT31880.7663701029118698No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT31640.7606006918485434No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA29910.7190128537670648No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA26340.6331928642000831No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT22630.5440073848461611No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT22060.5303050335707606No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA21200.5096313105938406No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT20930.5031407231475983No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT14900.3581842701815201No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC14820.35626113316041125No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT14730.35409760401166385No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT14730.35409760401166385No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC14610.3512128984800006No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG13450.3233274116739225No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC13420.3226062352910067No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12820.30818270763269046No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG12770.30698074699449746No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT12700.3052980021010272No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA12470.2997689831653393No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11920.2865474161452161No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG11730.2819799657200826No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT11610.27909526018841935No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC11320.27212388848689983No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG9960.23943055912804967No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9920.23846899061749524No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9090.21851644402349113No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG9040.2173144833852981No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8900.21394899359835762No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA8830.21226624870488742No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT8720.2096219353008628No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8670.20841997466266976No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8660.20817958253503116No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT8510.2045737006204521No Hit
CACGCTAGATACCATCTGGCAGAACATGGAGATGTCCTGGCCAT8500.20433330849281348No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG8240.1980831131742098No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8030.19303487849379908No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7990.19207330998324468No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC7970.19159252572796745No Hit
CAGTTCGCGGTTAGCCAGAACTTTCGCTTCTGCCAGTTCAATGG7960.19135213360032885No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7920.19039056508977442No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7680.18462115402644794No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC7540.18125566423950748No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7100.1706784106234089No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6890.16563017594299823No Hit
CGGTTCGCGCGGCTCACGTGGTACGCGCGGTTCACGCGGAACAC6810.1637070389218894No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6700.16106272551786474No Hit
CAGACGAGAACGTTTGGACAGAGAACGAGTGTAGGAAACGTTGC6660.1601011570073103No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6410.15409135381634523No Hit
CGGGTTCTGCATGATATCATCCATGATCACTACGCTCTGCTGGT6380.1533701774334294No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT6350.1526490010505136No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC6260.15048547190176614No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6080.1461584136042713No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA6070.1459180214766327No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6070.1459180214766327No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT6020.14471606083843966No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT5780.13894664977511315No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5740.13798508126455875No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT5710.13726390488164295No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5670.13630233637108852No Hit
GCGGAGCAGAGACAGGACGTTCTGCGGAACCTGGTTAGCCTGGG5650.13582155211581132No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT5320.12788861190373738No Hit
CTGGATCGGGGTCGGCTGAGATTCCTTCAGCAGAGCCAGCAGAG5300.12740782764846015No Hit
TTCGCGACCCTGTTCGCCCGGGTGGGTGTGGTTGTAACCAACGC5270.12668665126554438No Hit
ATCGTCGGACGGACGTTTGTTGTCGCTGCTAGTACGAGATGGAA5160.12404233786151972No Hit
CTGTTTTTTACGCGCGCCACGTGGTGCGGTATCGTGCGGCTGAC4940.11875371105347042No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG4930.1185133189258318No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC4920.11827292679819322No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG4760.11442665275597555No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4680.11250351573486672No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT4640.1115419472243123No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4580.11009959445848067No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4530.10889763382028765No Hit
CTGTTTTTTACGAGCACCGCGCGGTGCGGTATCGTGTGGCTGAC4510.10841684956501045No Hit
ACGCAGCAGAGACAGAACCGTCTGCGGGACCTGGTTTGCCTGCG4450.10697449679917881No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG4410.10601292828862441No Hit
GATACGCGGTACACGAGGTTCGCGCGGGGAACGTGGCACACGCG4330.10408979126751558No Hit
CGGGCCTTCACCTGGATCGTCCGCCGGACCCTGTTCGATAGCAC4230.10168586999112954No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4180.1004839093529365No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG4180.1004839093529365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)