FastQCFastQC Report
Wed 31 May 2023
EGAF00007938138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415987
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT99612.3945459834081353No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC57471.3815335575390577No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG42031.010368112465053No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG35890.8627673460949501No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA35080.8432955837562232No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC33400.8029097063129378No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT32200.7740626509963051No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA30130.7243014805751141No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT29900.7187724616394262No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG26180.6293465901578655No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC24410.5867971835658325No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG23710.5699697346311303No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA21920.5269395437838202No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15990.3843870120941279No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT15400.3702038765634503No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC15310.3680403474147029No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG15040.36154975996846056No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC14480.34808780082069873No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC14240.3423183897573722No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13990.3363085865664071No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13600.32693329358850154No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13520.32501015656739274No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA13120.31539447146184857No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12620.3033748650799184No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG12450.2992881989100621No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG12300.29568231699548303No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC12220.2937591799743742No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11620.27933565231605795No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10840.26058506636024686No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9950.2391901670004111No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA9930.23870938274513387No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9930.23870938274513387No Hit
GGCAAATCCCTGACCACCTCTATTGTTGGTC9920.23846899061749524No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9800.23558428508583198No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC9570.23005526615014413No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9350.22476663934209484No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9280.2230838944486246No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9240.2221223259380702No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC9090.21851644402349113No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8980.21587213061946647No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8680.20866036679030833No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8600.20673722976919953No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT8600.20673722976919953No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG8470.20361213210989765No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8360.200967818705873No Hit
ATCAAAGACCTGCAGGCACAGGTGGTAGAAA7970.19159252572796745No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA7850.18870782019630422No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG7530.18101527211186888No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7290.1752458610485424No Hit
CCTACCGATGGCCCGCCGCACCGTGGTGGCG7180.17260154764451774No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6920.16635135232591403No Hit
GGCGGTACCTACCGTAGCTATGAACAGCTGA6820.16394743104952797No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6820.16394743104952797No Hit
ATCCACGGTTCTCCGGGCACTGGTAAATCCC6710.16130311764550334No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6690.16082233339022614No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA6520.15673566722036988No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6180.14856233488065732No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT6130.1473603742424643No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6000.14423527658316246No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT5980.14375449232788526No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT5850.1406293946685834No Hit
CGTTTCAACAGCGCTATCACCAACCTGGGCA5590.1343791993499797No Hit
ATCCACGTTCAGTGCAACTCTTCCAAATTCC5580.1341388072223411No Hit
AACGCTAAAATCGCGGAGGCAGAAGCGCTGC5260.12644625913790575No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5210.12524429849971275No Hit
GGTCGTTCTGGCTATACTATCCATGTACAGT5190.12476351424443553No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5060.12163841658513366No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4860.11683057403236159No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4850.11659018190472298No Hit
CTGCGTGTCTGCGCTGCGCTGTCCATCCCGA4840.11634978977708439No Hit
GCCCAGTCCGCGACCCCGTCTGTTTCTTCCT4840.11634978977708439No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4480.10769567318209464No Hit
CTGGCCTCTTCTGCACCGTCCACCGCGGTTG4460.10721488892681742No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG4440.10673410467154022No Hit
CGCTTCGAATCCACCATCACCAACCTGAACA4420.106253320416263No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC4190.10072430148057511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)