FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007938139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390771
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG35530.9092281668803468No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT29680.7595241202648098No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26460.6771229185379672No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24010.6144263520066741No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC22890.5857650644495114No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA22730.581670594798488No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT22150.5668281423135288No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT17250.44143500925094237No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA16530.42300989582133786No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16310.41738000005118087No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA16230.41533276522566925No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14410.36875817294528No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA12510.3201363458393791No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12170.3114355978309547No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC11980.3065734151203646No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11650.29812857146512917No Hit
CAGACCCACGGATTCTTCGCTGGACAGGTGTTCACGGATGCGTT11420.2922427713417833No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11340.29019553651627167No Hit
CGCGAAGGTGTCCACCGGTTCAACTTCGCTCGGTGGCGGGGTCA11160.28558925815887054No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT11150.2853333538056816No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG11010.28175069286103627No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA11000.2814947885078473No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10510.26895547520158863No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC10220.26153424895910904No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC10180.26051063154635323No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG9730.24899493565285039No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT9550.24438865729544926No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8120.2077943347894291No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7600.1944873084236036No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7540.19295188230446988No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT7510.19218416924490303No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG7460.19090464747895827No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT7430.1901369344193914No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7140.1827157081769118No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6990.17887714287907752No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6790.1737590558152985No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6590.16864096875151943No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6060.15507803803250497No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5750.14714500308364747No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5640.14433005519856898No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5260.1346056897773888No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5090.13025531577317662No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4880.12488132435620862No Hit
CTGGCCGTCGATGTTACGGACCGGAATCAGCAGTGCACCATCAA4830.12360180259026388No Hit
CATGTGGTCACGCTGCATCAGACCGGCGTCCGGTTTCGGCAGAG4410.11285381975632788No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG4390.11234201104994997No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT4260.1090152544584936No Hit
CCAGTTACCATCTTCGGTGGTGCGTTCGACGTGGCAACGTGCCA4250.10875935010530464No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC4230.10824754139892674No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4210.10773573269254884No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG4200.10747982833935989No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA3950.10108221950963607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)