FastQCFastQC Report
Wed 31 May 2023
EGAF00007938140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390771
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56171.437414751862344No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA48491.2408802086132287No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35910.918952532301527No Hit
ATGTCCAACATGGACATCGATGGTATTAACA34200.8751928879062163No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG29190.7469848069585512No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT27950.7152526671631211No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23980.6136586389471071No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23470.6006075169344706No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23240.5947217168111246No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21230.5432849418201453No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19400.496454445186567No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18320.46881677504216024No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17440.44629719196153245No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17180.43964367877861965No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14540.37208492953673633No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12420.3178332066606785No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12310.3150182587756No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12050.3083647455926873No Hit
CCGGTAGCTCCGCAGCAGCAGTACCAGCAGC11840.3029907541757193No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11210.2868687799248153No Hit
CCGTGTAAAGACGCGAACGAATGCCATCTGA11010.28175069286103627No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10690.2735617535589898No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10660.27279404049942296No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10290.2633255794314317No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG8800.22519583080627786No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8260.21137699573407442No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8180.20932976090856284No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8010.20497938690435064No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7820.20011720419376056No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG7760.19858177807462685No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7690.19679044760230416No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7460.19090464747895827No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7280.18629836912155712No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT7130.18245980382372284No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6750.17273543840254266No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6370.16301107298136247No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6150.15738117721120554No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6100.15610165544526078No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6010.15379851626656021No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5750.14714500308364747No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5630.14407415084538003No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5560.14228282037305737No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4890.12513722870939756No Hit
GGTGGCGAAGATGAGACCCGTCAGCCAGCAC4870.12462542000301967No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4790.12257818517750806No Hit
CGCACCATCTGGGAATCCGCTGCCGATATCG4580.11720419376054006No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC4210.10773573269254884No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4070.1041530717479035No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA3920.10031450645006923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)