FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007938146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419772
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT45921.0939271795164995No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22500.5360052599982847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21490.5119445794383618No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC17570.41856055191866065No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC16120.38401799071877113No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC14340.34161401903890687No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC13270.3161239911189884No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12840.3058803350390212No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10870.25895009671917135No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10770.256567851119179No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10390.24751531783920797No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10320.24584774591921327No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9540.22726623023927275No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8680.2067789180793383No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG8430.20082330407935736No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7830.1865298304794031No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7320.174380377919442No Hit
CGCGATAACAACATTGAACGTGACAACAATA7130.16985411127945646No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6890.16413672183947478No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6770.1612780271194839No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6510.15508418855950373No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6480.154369514879506No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6270.14936679911952203No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6150.14650810439953119No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA5790.13793202023955864No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5700.1357879991995655No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG5620.13388220271957157No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5460.13007060975958376No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5450.12983238519958454No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5170.12316209751960588No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5090.12125630103961198No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5050.12030340279961503No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4810.11458601335963332No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA4720.1124419923196402No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG4660.11101264495964476No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG4550.10839217479965316No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4500.10720105199965696No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA4480.10672460287965849No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4270.10172188711967448No Hit
CCGGGCATCGGCAAACCGGGCGGTCCGGGTC4210.10029253975967906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)