FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007938179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499541
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22650.45341623610474413No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT20430.40897543945341824No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18190.364134275264693No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG18060.3615318862715973No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14060.2814583787917308No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13870.27765488718643716No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12560.25143081348678087No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11610.23241335546031255No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA11270.2256071073245239No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG10810.21639865396433927No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10790.21599828642693994No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10720.21459700004604226No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10690.21399644873994328No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8910.17836373791140267No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8780.175761348918307No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8710.17436006253740932No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7610.15233984798044606No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7560.15133892913694771No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7290.14593396738205672No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7120.1425308433141624No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA7120.1425308433141624No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7120.1425308433141624No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7070.14152992447066406No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6990.13992845432106674No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6880.1377264328653704No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6870.13752624909667074No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG6490.1299192658860834No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6230.12471448789989209No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6000.12011026121979977No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT5940.11890915860760178No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5780.11570621830840713No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5750.11510566700230812No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5720.11450511569620911No Hit
TTCGTGCAGAGGATATGCCATGCCCTGGGCCGCCAGATGGATGG5480.10970070524741714No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5010.10029206811853282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)