FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007938180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499541
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45710.9150400067261746No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30760.6157652725201735No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20270.4057724991542236No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19380.3879561437399533No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG18800.37634548515537264No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT16190.32409752152475974No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15660.3134877817836774No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13710.2744519468872425No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13460.26944735266975084No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13400.26824625005755287No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11660.2334142743038109No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC11640.2330139067664116No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10960.21940141049483428No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG10690.21399644873994328No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10520.21059332467204894No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10380.20779075191025362No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9610.1923766017203793No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9380.187772375040287No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA9300.18617090489068966No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9260.185370169815891No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8870.177563002836604No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8140.16294958772152837No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7610.15233984798044606No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7460.14933709144995105No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7290.14593396738205672No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7140.14293121085156174No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7110.14233065954546273No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7010.14032882185846607No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6970.1395280867836674No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6880.1377264328653704No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6730.13472367633487542No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6470.12951889834868408No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA6350.1271166931242881No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6210.12431412036249276No Hit
GCGCGCGGCTCCACCTACGGTACCCCGCGTC5920.11850879107020244No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5460.1093003377100178No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5440.10889997017261846No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT5410.10829941886651946No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5160.10329482464902781No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5080.10169335449943047No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5040.1008926194246318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)