FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007938181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503954
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA31600.6270413569492453No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28600.5675121142008993No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20790.41253765224603833No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT16980.3369355139556388No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC11990.23791854018422315No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG11960.23732324775673969No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG11140.22105192140552513No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT9940.19724022430618668No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9550.18950142274890167No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC9440.187318683848129No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT9050.179579882290844No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC8820.17501597368013747No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8790.174420681252654No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8070.16013366299305096No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7950.1577524932831171No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7830.15537132357318326No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7600.15080741496247674No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG7480.1484262452525429No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG7120.14128273612274136No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6800.13493295022958446No Hit
CAGTTCGCGGTTAGCCAGAACTTTCGCTTCTGCCAGTTCAATGG6640.131758057283006No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT6620.13136119566468368No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT6540.12977374919139445No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6470.1283847335272664No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5880.11667731578675833No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5690.1129071304126964No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5600.11112125313024603No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5380.10675577532870063No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA5250.10417617480960564No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5080.10080285105386602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)