FastQCFastQC Report
Wed 31 May 2023
EGAF00007938205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498659
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27120.5438586288425558No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA23940.4800875949296012No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT22770.4566246673578538No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC19900.39907030656219983No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16110.32306646425713764No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13800.2767422226411235No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13210.26491048993400296No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG12830.2572900519192474No Hit
TGGACGACCGCCGCCGCGACCACGACCGCGGCCACGACCGCGAC12640.25347983291186965No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC12190.2444556299996591No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12180.24425509215716554No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11560.23182174592256433No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11180.22420130790780876No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG10980.2201905510579374No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9900.19853246406863206No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9450.18950826115642153No Hit
CGGGCGACCGCCACCACGACCACGACCACGACCGCGGCCACGGC8540.17125931748950685No Hit
TGGGCGGCCGCCACCACGACCGCGACCGCGGCCACGACCACGGC8300.16644640926966123No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7880.1580238198849314No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7580.15200768461012434No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7410.1485985412877337No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7400.14839800344524015No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7350.1473953142327723No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7330.14699423854778515No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7090.14218133032793953No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6970.1397748762180167No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6440.12914637056585765No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6390.1281436813533898No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT6300.1263388407709477No Hit
AATACCGCGGCCCTGGTTGTACACGCCGTGGTGTTCATGTGCGT6070.12172647039359562No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5900.11831732707120499No Hit
CAGGGTAACGGTACCACGAGAGACGTGTTTCACCTGCAGTTTCT5860.1175151757012307No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC5750.11530925943380146No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5600.11230119179639796No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5330.10688667004907161No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5200.10427967809665523No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5010.10046945908927743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)