FastQCFastQC Report
Wed 31 May 2023
EGAF00007938206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498659
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT59371.1905931708843118No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27420.5498747641173628No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23770.47667845160721056No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17460.3501390729937693No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG14920.29920246100040304No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14690.294590090623051No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14560.29198309867063466No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14030.28135459301847554No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13830.2773438361686042No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA13760.2759400712711492No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13390.2685201710988872No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12980.26029811955665094No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12500.2506723031169597No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG12300.24666154626708836No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10750.21557818068058532No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10220.20494967502842623No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9610.19271686663631862No Hit
ATGAGCGACGAAGGTCCGGGTACCGGCCCGG9370.18790395841647298No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8680.1740668472844168No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8440.16925393906457117No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8270.16584479574218053No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8150.16343834163225773No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7850.1574222063574507No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7630.1530103738225922No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7550.15140607108264365No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7550.15140607108264365No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6910.1385716491630553No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6740.13516250584066467No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6570.13175336251827402No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6550.1313522868332869No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6470.12974798409333832No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6430.12894583272336407No Hit
CTGGACACCAACTCCGTTTATGAACCGTACT6380.12794314351089622No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6340.12714099214092195No Hit
ATTCCACAGGTTACCCGCAACGACACCGGCA6120.12272915960606345No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5910.11851786491369853No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5590.11210065395390438No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG5280.10588398083660377No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC5000.10026892124678387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)