FastQCFastQC Report
Sun 21 May 2023
EGAF00007938248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469570
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT57181.2177098196222076No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA54081.1516919735076772No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42170.89805566795153No Hit
ATGTCCAACATGGACATCGATGGTATTAACA30390.647187852716315No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25570.5445407500479161No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25320.5392167301999702No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24370.5189854547777755No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA24300.5174947292203506No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA18530.394616351129757No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15690.3341354856570905No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15610.3324317993057478No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC11510.24511787379943353No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11120.2368124028366378No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10760.22914581425559555No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10660.22701620631641714No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9250.19698873437400174No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG9140.1946461656409055No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8970.19102583214430224No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8050.17143343910386097No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8020.17079455672210747No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA7820.16653534084375068No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7630.1624890857593117No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7550.160785399407969No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7320.15588730114785868No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7100.1512021636816662No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA6520.1388504376344315No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA6480.13799859445876014No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6420.13672082969525312No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6260.13331345699256766No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6150.13097088825947142No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6050.12884128032029304No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5810.12373022126626487No Hit
GAAGTCTCCATTGCCGAACCGACCACTTACA5640.1201098877696616No Hit
AAAATTTCCGGCAGCCAGGCATCCTCCCACA5450.11606363268522266No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5260.11201737760078369No Hit
CTGCAGGCGCCGAAAACTCGCTCCACCACCA5220.11116553442511233No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5000.10648039695891987No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG4720.10051749472922034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)