FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007938678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523183
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA44640.8532387329098995No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT41350.7903544266537712No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32770.626358272344476No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32550.6221532427468017No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23560.4503204423691137No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21740.4155333793338086No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21010.40158032657788956No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19140.36583757499765857No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18680.3570452403843397No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18380.35131110911478397No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16370.31289242960876024No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC15940.30467350812239696No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14150.27045985821404744No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13240.25306632669639495No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG12930.24714105771785397No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12210.23337914267092014No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10640.2033705223602449No Hit
ATGGGTGCACAGGTTTCCACTCAGAAATCTG9580.18310992520781447No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9490.18138968582694773No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT9200.17584669226637714No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA8700.16628980681711752No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC8620.164760705145236No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8620.164760705145236No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8540.16323160347335447No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8130.15539495740496156No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7720.14755831133656866No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG7660.1464114850826575No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA7460.14258873090295365No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7380.1410596292310721No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC7290.1393393898502054No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6510.12443064854936035No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6250.11946106811574536No Hit
GTTACCCTGGATGCAGGTAACCGTTTCTCTA6070.1160205893540119No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6050.11563831393604149No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG5940.11353579913720438No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5780.11047759579344131No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5740.10971304495750052No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5610.10722825474069303No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA5590.10684597932272265No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG5560.10627256619576705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)