FastQCFastQC Report
Wed 31 May 2023
EGAF00007938681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450764
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18360.40730848071274545No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17380.38556761409518064No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA16290.36138644612258297No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12700.281743883717422No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12650.28063465582877073No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9780.21696497502018794No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG9710.21541205597607618No Hit
AACAGTCTGAATTGCCGCATCCACAGTGGAAACCGGCGGTGGGG9180.20365424035637272No Hit
GACGGTCTGGATAGCCGCGTCTACGGTGGACACCGGCGGCGGGG9070.20121393900133996No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT8360.18546290298249193No Hit
CACAGTCTGAATTGCTGCATCAACAGTGGATACCGGCGGCGGCG8170.1812478370056171No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7680.1703774036968347No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG7520.16682787445315067No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7410.16438757309811786No Hit
ACCGGTCAGCGGGTAGCGGAATTTTGCATCAACTACTTCCGCGT7380.1637220363649271No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT6990.1550700588334472No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6880.15262975747841442No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6830.15152052958976317No Hit
CTGCGGTGGGAAACCCGGACGCGGTGCCATACCCGGGTGCGGCG6630.14708361803515807No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5920.13133258201631007No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5920.13133258201631007No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5490.12179322217390919No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5460.12112768544071842No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4910.10892617866555447No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4780.10604218615506118No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT4670.1036018848000284No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4640.10293634806683763No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4620.10249265691137713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)