FastQCFastQC Report
Wed 31 May 2023
EGAF00007938710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550753
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62421.1333574215664735No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA49550.8996773508269588No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23660.42959366539991617No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21100.383111848687161No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15420.2799803178557357No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC15250.2768936347146543No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC13600.2469346512865114No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13520.2454820945142378No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11920.21643095906876586No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11870.21552311108609484No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11810.21443369350688965No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC11650.21152857996234245No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC11420.20735247924205585No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11120.2019053913460299No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT10870.19736615143267489No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9820.17830134379658397No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG9430.17122012953175017No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG9210.16722559840799778No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9170.16649932002186096No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9150.16613618082879258No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT8730.1585102577743562No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8490.1541525874575354No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8150.14797922117537263No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT7960.14452939884122284No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7800.14162428529667564No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG7740.14053486771747045No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7600.13799289336599163No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7590.13781132376945746No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7560.13726661497985487No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7080.12855127434621327No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6910.12546459120513187No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6560.1191096553264349No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6220.11293628904427211No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC6110.11093902348239591No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6030.10948646671012233No Hit
GTACACGGCCACACCCAGGGCCCGTGGGACC5750.10440251800716474No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5610.10186054365568593No Hit
GGTCTGCAATCTTCCTCTTACCCAGGTTATG5600.10167897405915174No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5540.10058955647994654No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5510.10004484769034394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)