FastQCFastQC Report
Sun 21 May 2023
EGAF00007938736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522594
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA49780.9525559038182604No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT31570.604101845792336No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31290.5987439580247764No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG25920.49598732476836704No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22280.42633478379009326No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG21400.4094957079491919No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG21250.40662541093085647No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19900.38079273776583733No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA19550.374095378056388No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18560.35515141773537395No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG17540.3356333980106928No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16310.3120969624603421No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC12660.2422530683475126No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12240.2342162366961733No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12050.23058052713961505No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11590.221778282950053No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG11140.21316739189504663No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10900.20857491666570993No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10890.20838356353115423No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10880.20819221039659852No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10610.20302567576359468No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10270.19651966918870098No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9390.17968059334779962No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9170.1754708243875743No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8950.17126105542734896No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8720.1668599333325679No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8700.16647722706345652No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8550.16360693004512109No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7890.15097762316444505No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7370.1410272601675488No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7260.13892237568743612No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7120.13624343180365636No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC6930.13260772224709813No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6830.13069419090154116No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG6820.13050283776698546No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG6820.13050283776698546No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6340.121317887308312No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6240.11940435596275503No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6240.11940435596275503No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG6140.11749082461719805No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6110.11691676521353095No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5920.1132810556569727No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5630.1077318147548575No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5350.10237392698729797No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5340.10218257385274229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)