FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424039
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT53041.250828343619337No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT42651.0058037114510694No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG28270.6666839606734286No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT27500.6485252535733742No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25240.5952282690978896No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG24410.5756545978082205No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT22730.5360356004990108No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22400.5282532974561301No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22170.5228292680626074No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT16900.39854824674145534No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG15990.3770879565323001No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15450.3643532788257684No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG15110.3563351484179521No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA13140.30987715752560496No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12410.2926617598852936No Hit
TGCGTCACGACGCTGCTGAATTTGCGGGTTGGTCTGCATTTTTT11440.2697865054865236No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10470.24691125108775372No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10450.24643959635788215No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9640.2273375797980846No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA9240.21790448520065372No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9090.21436707472661712No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA9080.21413124736168135No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG8630.20351901593957158No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8180.19290678451746185No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7480.17639886897195778No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA7340.17309728586285697No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG7240.1707390122134992No Hit
CGGGATTTCTTCGTAGTCGTTTTCATACCAATCCGGGTTGTGCA6960.1641358459952976No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6830.16107009025113256No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6740.15894764396671063No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6230.14692044835498622No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT6040.14243972842120653No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC6030.1422039010562708No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5890.13890231794716995No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5770.13607238956794068No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5580.13159166963416102No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5540.13064836017441792No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5510.1299408780796106No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5480.12923339598480327No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5480.12923339598480327No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT4990.11767785510295044No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG4940.11649871827827157No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4830.11390461726397809No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA4810.11343296253410654No Hit
CAGCAGAGTACCACCTTTAGCGTCCATAATGATATCACCCTGGG4810.11343296253410654No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG4580.10800893314058377No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4470.10541483212629027No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG4390.1035282132068041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)