FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424039
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT56651.3359620223611506No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG52861.246583451050493No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT42360.9989647178679321No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC31860.7513459846853708No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG28740.6777678468254099No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG28430.6704571985124009No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27830.6563075566162546No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG26440.6235275528901822No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA23620.5570242359782944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19590.4619858079091782No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG17440.41128292444798714No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17210.4058588950544643No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC16550.3902942889687034No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14850.35020363692962203No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13350.3148295321892562No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12500.2947842061697155No Hit
GCTCGTCAGCGCTACCAATCCGCATCTCCGG11900.2806345642735692No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11280.2660132676475513No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9450.22285685986430495No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9190.21672534837597485No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9120.21507455682142443No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9050.21342376526687404No Hit
ATGTCCAACATGGACATCGATGGTATTAACA8800.20752808114347976No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7860.18536030883951712No Hit
CAGCCGCAGCGCCGTAAACGTCGTGCGGCTC7440.17545555951221467No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA7350.1733331132277927No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7300.17215397640311386No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7120.16790908383426995No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7060.16649411964465533No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6700.15800433450696752No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6660.15706102504722444No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6400.15092951355889433No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6290.14833541254460084No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6180.14574131153030737No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6170.14550548416537157No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6070.14314721051601387No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6030.1422039010562708No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5830.13748735375755533No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5610.13229915172896833No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5430.12805425916012442No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5240.12357353922634473No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5230.12333771186140899No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5220.1231018844964732No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4950.11673454564320736No Hit
GCGCTGAAGGCGGGCGCAGTTGAAGCGGCGT4920.11602706354840002No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4850.11437627199384962No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4620.10895224260032686No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG4600.10848058787045531No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4390.1035282132068041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)