FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415134
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA50541.2174382247659792No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC40370.9724570861456783No Hit
ATGTCCAACATGGACATCGATGGTATTAACA37470.9026001242972148No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG31980.7703536689358135No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30760.7409655677443909No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA26310.6337712642183007No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25370.6111279731363849No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25000.6022151883488224No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22650.545606960644033No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20690.4983932898774853No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG20220.4870716443365275No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19670.47382291019285344No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA19410.4675598722340257No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15140.36470151806404677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14660.35313898644774944No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14350.345671518112224No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14250.3432626573588287No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13820.332904556119229No Hit
CCGGTAGCTCCGCAGCAGCAGTACCAGCAGC12850.30953860681129464No Hit
CCGTGTAAAGACGCGAACGAATGCCATCTGA12250.29508544229092293No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12010.28930417648277423No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11490.27677810056511876No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11220.2702741765309514No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10400.2505215183531101No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9430.22715556904517575No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9260.22306050576440375No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8840.21294329060014358No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8810.21222063237412497No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8600.20716202479199486No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8430.20306696151122286No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7970.19198620204560454No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7910.19054088559356738No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG7810.1881320248401721No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7670.1847596197854187No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT7160.17247442994310272No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6920.166693164134954No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6710.1616345565528239No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6510.15681683504603333No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6190.1491084806351684No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6070.14621784773109406No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6060.14597696165575452No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5870.14140012622430348No Hit
CGCACCATCTGGGAATCCGCTGCCGATATCG5270.12694696170393174No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5150.12405632879985738No Hit
GGTGGCGAAGATGAGACCCGTCAGCCAGCAC5130.12357455664917832No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC4710.11345734148491814No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4580.11032582250550425No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4400.10598987314939273No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4390.1057489870740532No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4350.10478544277269508No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT4340.10454455669735555No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4270.10285835416997885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)