FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508255
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA75931.493935130987398No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37670.7411633923916144No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA37590.7395893793469813No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA34090.670726308644283No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24060.473384423173407No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21770.42832829977078435No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19410.3818949149541077No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19280.3793371437565789No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG18030.3547431899341866No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15850.3118513344679344No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC15490.30476827576708543No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG14400.28332234803395934No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14080.2770262958554269No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14030.2760425377025312No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC13850.2725010083521067No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13800.271517250199211No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13390.26345043334546636No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12770.25125183224955977No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10730.21111449961141554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10590.2083599767833076No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10180.2002931599295629No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9840.1936036044898722No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG9830.19340685285929307No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9420.1853400360055484No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8970.1764862126294872No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8890.17491219958485404No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8770.1725511800179044No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8570.16861614740632164No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8350.1642876115335806No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8110.15956557239968128No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7660.15071174902362003No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7490.14736697130377469No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7310.14382544195335017No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6680.13143008922686447No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6180.12159250769790755No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6110.12021524628385356No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6090.1198217430226953No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5950.11706722019458736No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5880.1156899587805334No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG5760.11332893921358372No Hit
ATCCACGGTTCTCCGGGCACTGGTAAATCCC5750.11313218758300458No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA5570.1095906582325801No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5570.1095906582325801No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5410.10644263214331388No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5360.1054588739904182No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC5270.10368810931520596No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5260.10349135768462682No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5100.10034333159536059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)