FastQCFastQC Report
Sun 21 May 2023
EGAF00007940789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514350
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT116512.2651890735880236No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT25840.5023816467386021No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG24520.4767181880042773No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21450.4170312044327792No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC20310.39486730825313504No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG20270.3940896276854282No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA19340.3760085544862448No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG19270.3746476134927579No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC15080.2931855740254691No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14580.28346456692913385No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG12860.2500243025177408No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG12310.23933119471177214No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11270.21911149995139495No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10970.21327889569359385No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10850.21094585399047341No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG10750.20900165257120637No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10200.19830854476523768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10170.19772528433945757No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA10020.194808982210557No Hit
ATGATGGCTCGTCGCACCGTTGATTTCAAAA9730.1891707980946826No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9260.18003305142412754No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9170.17828327014678721No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC9100.1769223291533003No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG9040.17575580830174006No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8790.17089530475357248No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8040.1563137941090697No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7810.1518421308447555No Hit
CAGTCCATTGTAAACCGTACTGACCTGGATC7690.14950908914163508No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7160.1392048216195198No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7000.13609409934869252No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6790.13201127636823176No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA6730.13084475551667152No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6720.13065033537474482No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6650.1292893943812579No Hit
CCGCGTGTCCTGAGCAACTCCGGTTGGGGTC6420.1248177311169437No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA6350.12345679012345678No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6140.11937396714299602No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6090.11840186643336251No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5970.11606882473024205No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5870.114124623310975No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG5820.1131525226013415No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5730.11140274132400117No Hit
TACTATAACGGTAAAGTTCCGTATGATGCAC5700.11081948089822105No Hit
ATTTACACTGCATTCGCCTCTAACATCAAAG5620.10926411976280742No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5210.10129289394381258No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5210.10129289394381258No Hit
GAAACCGAACGTAACGTAGCCATCGACAAAG5190.10090405365995916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)