FastQCFastQC Report
Sun 21 May 2023
EGAF00007940791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520200
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT110882.131487889273356No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT26950.5180699730872741No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG26030.5003844675124952No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22410.43079584775086505No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG21610.41541714725105733No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG21060.4048442906574395No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC19230.36966551326412916No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA19010.36543637062668205No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC14730.2831603229527105No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13850.266243752402922No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG13140.25259515570934254No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG12420.23875432525951557No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12050.23164167627835447No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11140.21414840445982314No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA10830.20818915801614762No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10810.20780469050365244No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG10300.198000768935025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10210.1962706651287966No Hit
ATGATGGCTCGTCGCACCGTTGATTTCAAAA10130.19473279507881586No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9980.19184928873510187No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9530.18319876970396No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9460.18185313341022685No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9420.18108419838523646No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC9230.1774317570165321No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG9140.17570165321030373No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8040.15455594002306805No Hit
CAGTCCATTGTAAACCGTACTGACCTGGATC7980.15340253748558247No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7890.1516724336793541No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7140.13725490196078433No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA7040.13533256439830835No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6760.12995001922337562No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6730.12937331795463283No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6690.12860438292964244No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6680.12841214917339486No Hit
CCGCGTGTCCTGAGCAACTCCGGTTGGGGTC6520.1253364090734333No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6320.12149173394848135No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6260.12033833141099579No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA6020.11572472126105343No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5960.11457131872356786No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5950.11437908496732027No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG5900.11341791618608228No Hit
TACTATAACGGTAAAGTTCCGTATGATGCAC5840.11226451364859669No Hit
ATTTACACTGCATTCGCCTCTAACATCAAAG5560.10688196847366398No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5320.10226835832372165No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5310.10207612456747406No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5280.10149942329873125No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA5270.10130718954248366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)