FastQCFastQC Report
Wed 31 May 2023
EGAF00007940796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496216
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA43750.8816724974607831No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA40970.8256485079078465No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25780.5195318167894626No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22880.461089525529205No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT20540.4139326422364454No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG18980.38249472004127233No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18430.37141083721605106No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14730.2968465345736534No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG14570.2936221322972254No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14210.28636722717526236No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13320.2684314895126316No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA12140.24465152272397503No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11660.234978315894691No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11150.22470053363857675No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10610.2138181759556322No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG10250.2065632708336692No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT9650.19447176229706417No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9140.1841939800409499No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8520.1716994212197914No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8340.16807196865880986No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8310.16746739323197962No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8150.16424299095555162No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7840.15799571154497236No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7730.1557789349799281No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7690.1549728344108211No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7470.1505392812807326No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7350.14812097957341158No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG7110.14328437615876957No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7080.1426798007319393No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT6960.14026149902461832No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6810.13723862189046707No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6790.13683557160591356No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6740.1358279458945298No Hit
GTTCAGACCGAACTCTTCTGCAAATTTCTTGGCACCTTCCTGCA6210.12514711335386203No Hit
GCCGCGGGTAATCTGGATCAGACGGTCAGAGTAGCCGCAGGCTT6190.12474406306930852No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC6000.12091508536605028No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT5930.11950440937011302No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT5850.11789220823189901No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5680.11446628081319425No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT5630.1134586551018105No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5620.11325712995953374No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5290.10660680026440099No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5210.10499459912618697No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG5110.10297934770341947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)