FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496216
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT98041.9757524948812615No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31540.6356102987408709No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26020.5243684202041047No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21840.440130910732423No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21000.42320279878117595No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19940.40184113369984037No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19030.38350234575265607No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18770.3782626920534606No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15870.31982040079320295No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15460.3115578699598562No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13920.28052299804923664No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA13300.2680284392280781No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13110.2641994615248198No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13010.26218421010205234No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11960.24102407016299351No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10190.2053541199800087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9840.19830074000032244No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9340.1882244828864849No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9200.1854031308946104No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9030.18197720347590565No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8640.1741177229271124No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8090.1630338401018911No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8020.16162316410595387No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7640.15396520869943733No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7600.15315910813033035No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7540.15194995727666982No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7500.15114385670756283No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7480.15074080642300933No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG7470.1505392812807326No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7190.14489657729698358No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7030.14167217502055557No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6800.1370370967481903No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA6710.13522337046769956No Hit
CTGCGTTTCAAAACCGGCCGTAACGCTGCGT6490.13078981733761103No Hit
GACGCAGCTTCCTCTGGTGCGAGCCGTCTGG6490.13078981733761103No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6360.12816999048801328No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6030.12151966079288051No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5980.12051203508149676No Hit
ATGGGTGCACAGGTTTCCACTCAGAAATCTG5910.11910135908555952No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5800.11688458252051527No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5710.11507085624002451No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5570.11224950424814999No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5460.11003272768310575No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5060.10197172199203572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)