FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377960
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54871.4517409249656048No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23090.6109112075351889No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA17280.4571912371679543No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15760.4169753413059583No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15310.4050693195047095No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15050.3981902846862102No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG13420.3550640279394645No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13330.3526828235792147No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12680.33548523653296647No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12620.3338977669594666No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12070.31934596253571806No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11540.30532331463646945No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10280.2719864535929728No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9190.2431474230077257No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8770.2320351359932268No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8380.22171658376547781No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC8320.220129114191978No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7930.209810561964229No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7850.20769393586622925No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7360.19472960101598052No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC7300.19314213144248069No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7110.18811514445973118No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6750.17859032701873215No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6670.17647370092073233No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6550.17329876177373266No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6420.16985924436448302No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6010.1590115356122341No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6000.15874695734998415No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT5860.1550428616784845No Hit
CTGCCGGTTACCGTCACCATCGCCCCGATGT5800.15345539210498466No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5780.1529262355804847No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5420.14340141813948565No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5340.14128479204148586No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5220.13810985289448618No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4990.13202455286273682No Hit
TGTGAACGTTTCGGCGCCGCCGTCACTGACA4910.129907926764737No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA4820.12752672240448726No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4550.12038310932373797No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4460.1180019049634882No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4410.11667901365223833No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4410.11667901365223833No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4300.11376865276748861No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4240.11218118319398877No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC4070.10768335273573923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)