FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007940816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470742
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT50191.0661891227041564No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC46070.9786677203223846No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT42330.8992186802962132No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA39290.8346397814514107No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC33910.7203521249431748No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA32250.6850886472844998No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA26800.569313976658127No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT24860.5281024425269043No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC23260.49411354839806093No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT20030.4254984683754583No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18770.39873221424899413No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC17840.3789761695366039No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA17160.3645308895318455No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT15960.33903921893521294No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG15870.3371273436404655No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA13690.29081747538991637No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12510.26575066596989433No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10970.23303635537088255No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9420.20010961418356552No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9360.19883503065373387No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9090.19309940476949156No Hit
GCGCAGCGGTGCGTCCTTATCACCGGTCGGCGCCGGAATGTCGG9010.19139996006304938No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8980.1907626682981336No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG8840.18778864006185977No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8710.18502704241389126No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8490.18035356947117528No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7870.16718287299624848No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG7710.16378398358336413No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7500.15932294122895344No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7480.1588980800523429No Hit
GTCTTCCGGGCCTTCCTCACCCGGGCGACGGTGCGCGCGGTGAC7420.15762349652251126No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7030.14933870357860568No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6500.1380798823984263No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG6380.13553071533876304No Hit
ACGAACGATTACTTTCTGGCGGTCCACGATGTACAGGCCTTTGC6340.13468099298554198No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6330.1344685623972367No Hit
GTTGCACGCAGCGTTCCAGGTCTTTTCGAACGCATCAGTGCTGT6210.13191939533757344No Hit
GTGACGACGGCGGCGTTCACCGCCGTCCGGACCTTCGCCTTCAC6030.12809564474807858No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6000.12745835298316274No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5880.12490918592349949No Hit
ACCCTCACCGGAACGAGCCGGACGAGAACCTTCACGGTGACGGC5430.11534980944976228No Hit
GTCTGGGCCATCTTCACCCGGGCGACGATGTACACGGTGACGGC5370.11407522591993066No Hit
TTTCACGACCACTTTAACGGTTTCGTCACCAACAGTTACCAGTA5260.11173848944857268No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5260.11173848944857268No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA4940.10494071062280401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)