FastQCFastQC Report
Wed 31 May 2023
EGAF00007940817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470742
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA84681.7988622217690369No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC59291.2595009580619532No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT50781.0787225274141674No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA39820.8458986026315901No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA37390.7942779696734092No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT37000.7859931767295038No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG34250.7275747649455541No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA28980.615623844908676No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA25560.5429725837082733No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC22450.47690667074533394No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19680.4180633977847738No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG19480.4138147860186684No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA15680.3330911624626653No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT15050.3197080353994332No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14310.30398817186484317No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13350.28359483538753716No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12290.26107719302717836No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10470.22241482595561898No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10260.2179537836012083No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10250.21774135301290304No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9800.20818197653916584No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9490.20159662830170239No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9260.19671072477068116No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9210.19564857182915482No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9150.1943739882993232No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9130.19394912712271264No Hit
GTTGTGAACAAAATTGATCTGCCGTCTGCTG9110.19352426594610211No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC8940.1899129459449125No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8600.1826903059425333No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8530.18120329182439637No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7860.1669704424079432No Hit
GGCGAAGCTGAACGTGGCAAAGCCGGTGATC7430.15783592711081654No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG7330.15571162122776383No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC6670.1416912023996159No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6670.1416912023996159No Hit
ACCGCCACCGTTAAAGCATATGGCGGCGAAG6610.1404166188697843No Hit
ACCAAACAGTATCATCGCTCCTCTCGTAACC6600.14020418828147904No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6600.14020418828147904No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6290.1336188400440156No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5900.12533404710011004No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5690.12087300474569936No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA5600.11896112945095189No Hit
GCTGAACGTGGTAAAGCAGGTGACCCGCACC5390.11450008709654119No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5250.11152605886026741No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5150.1094017529772147No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5100.10833960003568832No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5010.1064277247409409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)