FastQCFastQC Report
Sun 21 May 2023
EGAF00007940825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369550
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56061.5169801109457448No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28220.7636314436476797No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17850.4830198890542552No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17680.4784196996346909No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15870.42944121228521176No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15870.42944121228521176No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT12830.34717900148829656No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA11960.32363685563523203No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11760.3182248680828034No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10740.29062373156541743No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10610.2871059396563388No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10210.2762819645514815No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9660.26139899878230277No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9530.2578812068732242No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG9290.25138682181030986No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC9060.24516303612501694No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC8180.22135029089433095No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7340.19861994317413068No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7250.19618454877553781No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7240.1959139493979164No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6740.1823839805168448No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6740.1823839805168448No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6660.18021918549587337No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6480.1753483966986876No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6450.1745365985658233No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6340.17156000541198754No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6320.17101880665674468No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6190.1675010147476661No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5740.15532404275470166No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG5540.14991205520227302No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5480.14828845893654446No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5420.14666486267081585No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5330.14422946827222297No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5100.13800568258693005No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5100.13800568258693005No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4860.1315112975240157No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4790.1296171018806657No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA4740.1282641049925585No Hit
TCTATCGTAGTGACCGGCAACGCAGGTTCCG4620.12501691246110133No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4580.12393451495061561No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4440.12014612366391558No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4420.11960492490867272No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4370.11825192802056556No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4350.1177107292653227No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4090.11067514544716547No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA3980.10769855229332972No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA3900.10553375727235827No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA3810.10309836287376538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)