FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407278
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC54161.3298042123561793No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA44831.1007223567194888No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG37000.9084703814102406No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT33710.8276901772253842No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22380.5495018144854374No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21920.5382073178516885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20040.49204720117462764No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18860.4630743619837065No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18820.46209223184164133No Hit
GCTGAACGTGGTAAAGCAGGTGACCCGCACC16120.3957984472522454No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT15770.38720480850917555No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15420.3786111697661057No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13760.337852768870403No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13640.3349063784442077No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT13500.3314689229469797No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13210.32434847941700756No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13060.3206654913842633No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12870.31600037320945396No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12560.3083888646084493No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12470.3061790717888027No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA12170.29881309572331427No Hit
GGCGAAGCTGAACGTGGCAAAGCCGGTGATC11660.28629093641198394No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11620.28530880626991884No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11520.28285348091475604No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10290.2526529790462534No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10170.24970658862005804No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC10150.24921552354902546No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9840.24160401494802078No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9600.23571123409563002No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9240.2268720628170439No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8640.21214011068606703No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8510.20894818772435536No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8180.20084561405231807No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC7940.19495283319992732No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7870.19323410545131336No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7800.19151537770269939No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT7730.1897966499540854No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7710.18930558488305285No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG7670.18832345474098774No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7660.18807792220547145No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7640.18758685713443887No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7580.18611366192134118No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7380.18120301121101556No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7230.17752002317827134No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC7100.1743281002165597No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7040.172854905003462No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6920.16990851457726663No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6760.16597999400900615No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT6600.16205147344074564No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT6580.16156040836971308No Hit
AACACTTCTAGCGGTCAGACCCAATCTACCA6420.15763188780145257No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6180.1517391069490618No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6050.14854718398735017No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5660.1389714151022152No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5550.1362705572115361No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5410.13283310171430815No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5370.13185097157224304No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG5350.1313599065012105No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5310.13037777635914535No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT5050.12399393043572204No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5040.12374839790020575No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4820.11834668211884757No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4670.11466369408610336No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC4390.10778878309164748No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT4310.10582452280751722No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4290.10533345773648467No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4280.10508792520096838No Hit
GCCGATGGCGAACCGCGTCGCCACCGTGTCC4170.10238706731028929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)