FastQCFastQC Report
Wed 31 May 2023
EGAF00007940832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452152
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23970.5301314602169183No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA22620.5002742440595198No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21270.47041702790212137No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA20740.45869530600329095No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18120.4007501902015252No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT15660.3463437074258214No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13520.29901449070224173No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12830.2837541357773492No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11150.24659848900369785No Hit
GCGAATGTGACGAGTCAGTTCATCAGAACGGGAGAAGCGACGGT10070.22271271607777915No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT10060.2224915515136503No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT9140.2021444116137936No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8860.19595180381818506No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC8820.19506714556166954No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8590.18998036058670537No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8590.18998036058670537No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8500.18798987950954546No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT8240.18223960084219465No Hit
ATGACGGGTCAGTTCGTCAGAACGGGAGAAACGACGATCGCAAC7710.17051787894336418No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG7540.16675808135317327No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT7460.16498876484014224No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6400.1415453210424813No Hit
TCAGCATTTACCGAAGTGAGACAGGGTCAGGGTGCCGTTAGATT6330.13999716909357915No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6180.136679700631646No Hit
ACCTGCACCAACTACGACCGGTTCCTTCAGCGGAGTGTGCAGTT5950.13159291565668182No Hit
AGGGTAAACGTCCGGCATTTCGTTTGCCGGTTTTACACGTGGCT5810.12849661175887755No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5800.12827544719474865No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT5770.12761195350236204No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5620.12429448504042888No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5470.12097701657849573No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5330.11788071268069145No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5240.11589023160353155No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC5180.1145632442187583No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG4970.10991878837205188No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4910.10859180098727861No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4820.10660131991011872No Hit
GCATTTACCGAAGTGGGACAGGGTCAGAGTACCGTTGCTTTCCA4560.10085104124276792No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT4550.10062987667863903No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4540.10040871211451016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)