FastQCFastQC Report
Sun 21 May 2023
EGAF00007940833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452152
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT51591.1409879863408765No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29380.6497814894106406No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23770.5257081689343407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21280.47063819246625027No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20290.4487429006174915No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18600.4113660892797112No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA17320.383057025071215No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15900.35165165696491446No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15020.33218917532157327No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11920.2636281604416214No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10400.2300111466940321No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10370.22934765300164547No Hit
CTGAACACCTCTTACCAGAGCCAGCTGATCA10370.22934765300164547No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9870.2182894247952016No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9380.20745236115288665No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA9220.2039137281268246No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8950.19794228489534493No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA8550.18909570233018985No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7830.1731718537129107No Hit
GCTGCCGCGTACAACCCGCACCACCTGCCTC7810.17272952458465296No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7730.17096020807162193No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7580.16764273960968878No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7100.15702684053150268No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6550.14486278950441445No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6360.14066066278596578No Hit
ATGTCTCTGGCAACTGATATCCTGAAACACG6220.13756435888816151No Hit
GTGAGCGTAGACTGCTCCGAATACCCAAAAC6180.136679700631646No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6060.13402572586209946No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6050.1338045612979706No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG5820.12871777632300643No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5790.1280542826306198No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT5310.1174383835524337No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5310.1174383835524337No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5260.1163325607317893No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5240.11589023160353155No Hit
GGCGCTCCGGGCCCGGCAGGCCCGCGTGGCG4690.10372618057644332No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4670.10328385144818557No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4670.10328385144818557No Hit
GCTCAGCACGATGAAGCACAGCAGAACGCGT4670.10328385144818557No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4580.10129337037102566No Hit
GAAGCGCAGAAACTGAACGACTCTCAGGCCC4540.10040871211451016No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC4530.1001875475503813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)