FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508697
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA106852.1004645201367413No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA56691.1144158506930453No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA51581.0139631253968473No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC24430.4802465907996312No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG22980.45174239281930106No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21840.4293321957864898No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG21460.421862130108886No Hit
GAATGCGACAACTATCGCCAGGAATACGATA20380.4006314171304332No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG20030.3937510934800087No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14470.2844522377761221No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC13350.2624352020947637No Hit
GCTAAAACCGCAGATACCCCGGCGACCTCTA13240.26027281466177316No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT12130.2384523596561411No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA12090.23766603695323543No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10180.2001191278894902No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG9950.19559777234778264No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9820.19304222356333928No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC9630.1893071907245374No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG9600.18871744869735815No Hit
TCCTACACCAACGGCAAACTGACTATCACCC9300.18282002842556572No Hit
ATCGAAGGTACTGACGATAAATGCGAGGAAG9240.18164054437120722No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9170.18026447964112233No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8800.17299099463924497No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG8200.1611961540956601No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8160.16040983139275444No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8140.1600166700413016No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7960.15647821787822613No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7710.15156370098506577No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7280.14311073192882995No Hit
ATGCAGCAGCAGGCATACATTAACGCGACCA7120.1399654411172073No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6940.13642698895413183No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6790.1334782788182356No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6780.1332816981425092No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6470.12718769719499032No Hit
GTTGAAACCCCGCAGCCGACCGCTGTATCCG6110.12011079286883941No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5810.11421337259704697No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5730.11264072719123563No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5440.10693988759516962No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5360.1053672421893583No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5340.10497408083790546No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5240.10300827408064132No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5130.10084588664765076No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5120.10064930597192434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)