FastQCFastQC Report
Fri 2 Jun 2023
EGAF00007940885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529736
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT83721.5804098645362974No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG53831.0161665433347933No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG51830.9784118881858133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42410.800587462434118No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG26370.4977951281392996No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT24990.4717444160865034No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24350.4596629264388299No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18220.3439449084072066No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17300.32657776703867586No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG15490.2924098041288491No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG15470.2920322575773593No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15340.28957820499267556No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA15170.28636905930501233No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14980.28278236706585924No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14340.27070087741818566No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14260.2691906912122265No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13980.2639050394913693No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13370.2523898696709304No Hit
TCCTACACCAACGGCAAACTGACTATCACCC13360.2522010963951855No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA12010.2267167041696241No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10900.2057628705619403No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG10540.1989670326351239No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9710.18329885074829724No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9550.1802784783363789No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9420.1778244257516952No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9140.172538774030838No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG8870.16744189558572573No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8830.16668680248274612No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8700.1642327498980624No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8540.16121237748614403No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8180.15441653955932766No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA7770.14667683525378677No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7640.1442227826691031No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA7490.14139118353292962No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7470.14101363698143982No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7320.13818203784526634No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7320.13818203784526634No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA7320.13818203784526634No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7220.13629430508781734No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7180.13553921198483773No Hit
GATGGTGAACGTAACAACCAGGTTCTGGAAG7020.13251883957291935No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC6960.13138619991844994No Hit
ATCCTGGCATTCACCCTGTACACTGAAGCTA6790.12817705423078665No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6650.12553422837035805No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG6560.12383526888865397No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6340.11968225682226619No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6270.1183608438920519No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC6170.1164731111346029No Hit
AACCTGTCCTGGACCGCCAAAGCGGACAAGT6100.11515169820438861No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5720.10797831372608242No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC5720.10797831372608242No Hit
AAAGCGATCAACGATGCTGCAACTTTCGTTA5670.10703444734735794No Hit
GCTCTGGAAGGCATCTCTAAAGACCAGGAAG5410.10212634217799053No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5390.10174879562650076No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5390.10174879562650076No Hit
ATGGCATCCACCACCCCGATCACCATGGAAG5330.10061615597203134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)