FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555559
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT86901.5641903020201273No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25640.4615171385937407No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG23220.4179574086640663No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG21920.39455755374316676No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG21670.3900575816429938No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21150.38069763967463405No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT19700.35459780149363074No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT18340.3301179532686897No Hit
CTGTCCTCCATGGCTATCACCTTCAAACGCG18180.32723797112457903No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC17950.3230979967924199No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA17820.32075801130032994No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC15330.2759382891826071No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT13350.2402985101492371No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA12610.22697859273272505No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12570.22625859719669736No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12370.222658619516559No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12360.22247862063255208No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC12050.21689865522833757No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11480.20663871883994317No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11250.20249874450778407No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10400.18719883936719592No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT10150.18269886726702295No Hit
TCTGTGCGCGCAGCTGTATTCGCCACCGCGG9830.17693890297880152No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9760.1756789107907531No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9700.17459891748671158No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9670.17405892083469082No Hit
GTTCTGGTTAACGAAGGTAACGGTTATGTGG9110.16397898333030336No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8850.15929901234612345No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8760.15767902239006118No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8420.15155906033382593No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8280.14903907595772906No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8110.14597909492961145No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7570.1362591551932378No Hit
CTGTCTTCCATGGCTATTACCTTCAAGCGTG7480.13463916523717553No Hit
GTCGCGGGTAAACGTGAAGGTCTGGGCGGTC7300.131399185325051No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7130.12833920429693335No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7100.1277992076449126No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7010.12617921768885032No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT6830.12293923777672577No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6820.12275923889271886No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA6720.12095925005264968No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6680.120239254516622No Hit
CTGTCCTCTATGGCAATCACCTTCAAACGTG6610.11897926232857356No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA6570.11825926679254589No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6490.11681927572049053No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT6450.11609928018446285No Hit
GAACTGGCTCGCCGTCAACAGGAAGAACGTA6430.11573928241644903No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA6130.11033931589624145No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC6010.10817932928815842No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6000.10799933040415148No Hit
CCGGCTCTGGGCGCTCGTCGTAAGAAAAAAA5950.10709933598411689No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC5790.1042193538400062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)