FastQCFastQC Report
Sun 21 May 2023
EGAF00007940901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519686
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA92891.787425483849863No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA55991.077381341810247No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA54651.0515965409882122No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42550.818763638042972No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG19930.3835008062560854No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA19460.37445688357970003No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18320.3525205604922973No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG16950.3261584880100676No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16810.32346455359582515No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA13820.26592981146307576No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA13110.25226771550513194No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12670.2438010644889414No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12600.24245409728182016No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC11460.2205177741944174No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC11250.21647687257305373No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10950.21070415597110562No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10850.2087799171037896No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC10290.1980041794468198No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9840.18934510454389766No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9320.17933906243385428No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8590.16529211870244725No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG8570.16490727092898402No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8360.16086636930762035No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC8160.1570178915729883No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7980.15355426161181945No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7860.1512451749710402No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG7840.150860327197577No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7640.14701184946294493No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7570.14566488225582372No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7390.14220125229465486No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7350.14143155674772842No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT7280.1400845895406072No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG7210.138737622333486No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7140.13739065512636475No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7100.13662095957943837No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA7040.13546641625904873No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6870.13219521018461147No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6840.13161793852441667No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6830.13142551463768506No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6630.127577036903053No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6620.1273846130163214No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA6560.12623006969593178No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG6470.12449825471534735No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6400.12315128750822611No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6090.11718614701954641No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6060.11660887535935162No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG5750.11064373487067192No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG5720.1100664632104771No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5700.1096816154370139No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5510.10602556158911343No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA5500.10583313770238183No Hit
GTGGAAGCGTGGATCCCGCGCGCTCCGCGTG5400.1039088988350658No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5310.10217708385448136No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5280.10159981219428654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)