FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007940905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527085
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT65441.2415454812791107No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41610.7894362389367939No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33870.6425908534676571No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22650.4297219613534819No Hit
ATGTCCAACATGGACATCGATGGTATTAACA19960.3786865496077483No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19200.3642676228691767No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18290.3470028553269397No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17050.3234772380166387No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15550.29501882997998424No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14750.27984101236043524No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT14570.2764260033960367No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13930.2642837493003975No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13890.26352485841942No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13070.24796759535938226No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT11570.21950918732272784No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11080.21021277403075406No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA10620.20148552889951338No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10210.19370689736949448No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10030.19029188840509595No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10000.18972272024436287No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9410.17852907974994547No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC9100.17264767542237022No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8980.17037100277943784No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8750.1660073802138175No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8390.15917736228502044No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8380.15898763956477607No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8140.15443429427891137No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8080.1532959579574452No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8050.15272678979671211No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8020.152157621635979No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7820.14836316723109177No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7720.14646594002864813No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7100.13470313137349765No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7040.13356479505203145No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT7020.1331853496115427No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6570.1246478272005464No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC6400.12142254095639222No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6180.11724864111101625No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6160.11686919567052753No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6130.11630002750979444No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5770.10947000958099738No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5540.10510638701537703No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5370.10188110077122285No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT5290.10036331900926795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)