FastQCFastQC Report
Wed 31 May 2023
EGAF00007940923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403860
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57711.4289605308770366No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT34620.8572277521913535No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA24130.5974842767295598No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG13840.34269301243005No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG10840.2684098449957906No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT10580.2619719704848215No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8970.2221066706284356No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8280.20502154211855592No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8060.1995741098400436No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG7700.19066012974793245No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7580.18768880305056207No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7240.17927004407467934No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7160.17728915960976574No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC6930.1715941167731392No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT6590.16317535779725648No Hit
GGTACCACCGTCTACCACAAAGTTCGTAAAG6480.1604516416580003No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6420.1589659783093151No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6290.15574704105383055No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA6280.15549943049571632No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG6280.15549943049571632No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6120.15153766156588916No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5760.14262368147377805No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5410.1339573119397811No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5350.13247164859109595No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5200.12875749021938296No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5100.12628138463824096No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5100.12628138463824096No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4990.1235576684989848No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG4820.11934828901104344No Hit
AAAATTTCCGGCAGCCAGGCATCCTCCCACA4690.11612935175555886No Hit
GAGAAACCGTACGATCCGTTCGACCGCAGCC4660.11538652008121628No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA4660.11538652008121628No Hit
CCGTACGATCCGTTCGACCGTTCCCACCTGA4640.11489129896498787No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT4580.11340563561630268No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4550.11266280394196009No Hit
TACATCCTGAAAAAAGGTGAAGAACCGTACG4490.1111771405932749No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4310.10672015054721934No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4200.10399643440796315No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4150.10275838161739217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)