FastQCFastQC Report
Sun 21 May 2023
EGAF00007940925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438734
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACGTCCGGTGTACCCGAAACCGGTTC48871.113886774218547No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT47581.0844839925786467No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA44711.0190685016433647No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG27690.6311341268285567No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21290.48525986132827637No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA18620.42440294118987815No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18260.41619751375548736No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17810.40594072946249893No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17500.3988749447273291No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG16680.38018480446010566No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16470.3753983051233777No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15070.3434883095451914No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14070.32069545556077256No Hit
CGTGTTAACAGCAACGTGCTGTACGGTACCG13360.30451252923183525No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13160.2999539584349515No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12190.27784489007006524No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC11130.2536844648465813No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG10970.2500376082090743No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10720.24433939471296956No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10620.2420601093145277No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC10580.24114839515515096No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC10170.23180332502153925No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG8110.1848500458136365No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7990.18211490333550626No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7930.18074733209644112No Hit
CCGTACGATCCGTTCGACCGTTCCCACCTGA7840.17869597523784345No Hit
ATGGCAATTAAACACACCACTATCGACATCG7740.17641668983940156No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7440.16957883364407592No Hit
GAGAAACCGTACGATCCGTTCGACCGCAGCC6770.15430762147451532No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5780.13174269602994068No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5750.13105891041040812No Hit
TACATCCTGAAAAAAGGTGAAGAACCGTACG5670.1292354820916546No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5590.12741205377290113No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5470.12467691129477088No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5360.12216969735648478No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5250.11966248341819873No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5190.11829491217913359No Hit
ACCGACCTGATTAGCGAAAAATACTACATCC4990.11373634138224983No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4940.11259669868302889No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4880.11122912744396378No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4850.1105453418244312No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG4800.10940569912521027No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG4700.1071264137267684No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA4530.10325162854941719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)