FastQCFastQC Report
Wed 31 May 2023
EGAF00007940930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440924
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA49451.121508468579619No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA43280.9815750560187244No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT41460.9402981012600811No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC37220.8441364044597255No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC33740.7652112382179241No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG33340.7561393800292113No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT27650.6270921972947718No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC20250.4592628208035852No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG19940.4522321307073328No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA17670.4007493354863877No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT15190.3445038147163684No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG14650.3322568061616061No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13640.30935036423510626No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG12950.2937014088595767No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG12450.2823615861236857No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC12430.2819079932142501No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG11660.26444466620097795No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA11600.26308388747267103No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9890.22430169371592382No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT9770.22158013625931No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9210.20887953479511207No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9200.20865273834039427No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8820.20003447306111713No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT8600.19504495105732508No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7970.18075677441010243No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG7950.1803031815006668No Hit
CACGCAGGCACCACGACGGCGACGAGACGGCGGCGGAGTAGGTT7500.17009734103836488No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT7180.16283985448739466No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7160.16238626157795902No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC7150.1621594651232412No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA7040.15966470412134517No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG6680.15150003175150367No Hit
TACGCAAGCGCCACGGCGACGACGGCTCGGCGGCGGGGTCGGTT6220.14106739483448394No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG6200.1406138019250483No Hit
AGACAGGCTTTTACGAGTGGACGCAACCTGTTTACCGACAGCGT6160.13970661610617702No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG6050.13721185510428102No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT6010.13630466928540974No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC5720.12972757209859295No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5720.12972757209859295No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5650.12813999691556824No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT5380.12201649263818709No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5220.11838774936270195No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5050.11453220963249904No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG5050.11453220963249904No Hit
GGAAGGCGGCGGGGTCGGTTTCACCGGCACCGGTTTCGGAACTG5000.11339822735890992No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA4580.1038727762607615No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA4550.10319238689660805No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC4530.1027387939871724No Hit
GGAGCTCGGACCTACGTTGGTAACAGTGAACACGTCCGGACGCG4420.10024403298527637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)