FastQCFastQC Report
Wed 31 May 2023
EGAF00007940931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440924
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT111872.5371719389282505No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA83931.903502644446662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63101.4310856292694434No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA43880.9951828433017935No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA43120.9779463127432392No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG32890.7459335395669094No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC26790.6075877021890393No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC18120.4109551759486896No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15330.34767896508241786No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14890.33769992107483376No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC13230.30005170959167565No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13030.29551578049731925No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12850.29143344431239854No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA12220.2771452676651759No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC10740.2435793923669385No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10260.23269316254048317No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10260.23269316254048317No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA9730.22067295044043872No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9630.21840498589326052No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9290.21069390643285463No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9250.20978672061398337No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8870.20116845533470618No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8670.1966325262403498No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7920.1796227921365133No Hit
GACAAAGCGGAGGCCCAGTCTACCCCGGAAC7610.1725921020402609No Hit
GACAAAGCGGAAGCTCAGAGCACCCCGGAAC7380.16737578358175106No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC7370.16714898712703324No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA7360.1669221906723154No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA7040.15966470412134517No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6850.15535557148170662No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA6210.14084059837976615No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6190.14038700547033048No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC6170.13993341256089484No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG6160.13970661610617702No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5970.13539748346653846No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5810.13176874019105334No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5650.12813999691556824No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5440.123377271366494No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5400.12247008554762272No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5320.12065571390988016No Hit
GCTGAAGCGCAGTCTACCCCGGAGCGTCCGG5310.12042891745516235No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG4900.11113026281173173No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA4880.11067666990229609No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT4830.10954268762870699No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT4820.10931589117398915No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT4770.10818190890040007No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4720.10704792662681097No Hit
GAAGCCGAGCTGGTTAACGACACCACCGCTC4520.10251199753245456No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA4450.10092442234942985No Hit
CACAAAGCAAAACACATCAAGGCCTGGTGCC4430.10047082943999419No Hit
CCGGAAAAACTGCCGAAAACTATCGAGAAAC4410.10001723653055856No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4410.10001723653055856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)