FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429503
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA31190.7261881756355602No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28680.6677485372628363No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23550.5483081608277475No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22540.5247926091319501No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17950.4179249039005548No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG15250.355061547882087No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14850.34574845810157323No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA14640.34085908596680353No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12880.29988149093254296No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG12680.2952249460422861No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12310.2866103379953108No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10370.24144185255981915No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8990.20931169281704665No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8960.20861321108350817No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8660.20162839374812283No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8600.20023143028104576No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG8400.1955748853907889No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8300.19324661294566045No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8230.19161682223407056No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT8020.18672745009930083No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7900.1839335231651467No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC7620.17741436031878707No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7200.16763561604924762No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6890.16041797146934947No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6360.14807812751016874No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6220.1448185460869889No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6190.14412006435345037No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5980.13923069221868065No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5860.13643676528452653No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5850.13620393804001368No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5780.1345741473284238No Hit
CGGCGGGATTGCAATGAAGGACGGACGCGGACGAGGGCGGCGTT5740.13364283835037238No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5680.13224587488329534No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT5650.1315473931497568No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC4880.11361969532226782No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG4650.10826466869847241No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC4640.10803184145395958No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4620.10756618696493389No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4310.10034854238503572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)