FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007940936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395667
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA31870.805475311309763No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA27690.6998309184238262No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG24390.6164274503559812No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG19330.48854213265195223No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG18570.4693340612181456No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG18470.4668066833979079No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG16850.42586316271005664No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG16590.41929198037743864No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG14310.36166776607601847No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG14000.3538328948332815No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC12290.3106147341072164No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11400.28812107150710065No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11130.2812971513924588No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10810.2732095423676981No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG10250.25905622657436683No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10100.25526515984401027No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG10080.2547596842799627No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9900.2502104042035348No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG9410.23782625288436993No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT9390.23732077732032242No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG9130.2307495949877043No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8330.2105305724258025No Hit
GGAGCTCGGACCTACGTTGGTAACAGTGAACACGTCCGGACGCG8250.20850867016961233No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG7750.19587178106842373No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA7730.19536630550437617No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG7730.19536630550437617No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG7530.1903115498639007No Hit
GATGCTGATGGAGCGACGAATGCGGAAACCTTGCGGAGTACGAG7310.18475131865937772No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7220.18247667862116373No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7200.18197120305711623No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7160.18096025192902113No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7090.1791910874548547No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT7050.17818013632675964No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG7030.17767466076271207No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT6690.16908157617390382No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6490.16402682053342837No Hit
GTCTTCTTTGGTTACGTCCTTAACAGTCAGGTTCTGACGACCGC6330.159983016021048No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6010.15189540699628729No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5900.1491152913940258No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5790.14633517579176428No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT5510.13925851789509866No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG5390.1362256645108134No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG4630.11701759307700668No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC4620.1167648552949829No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA4490.11347926412867387No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4410.11145736187248367No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT4060.10261153950165164No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC4030.10185332615558033No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT3970.1003368994634377No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG3960.10008416168141392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)