FastQCFastQC Report
Sun 21 May 2023
EGAF00007940937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395667
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT72151.823503097301519No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58101.4684065135581184No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG26010.6573709710438324No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC25200.6368992106999067No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG20770.5249363732633755No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG19270.48702570595980965No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG18590.4698395367821931No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC17910.45265336760457653No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16490.4167646025572009No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG16330.41272079804482054No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG15710.3970510555593466No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC12420.31390032527352546No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11680.29519772940376626No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11490.2903957115453146No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11300.28559369368686294No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10780.2724513290216268No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10650.2691657378553177No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG10370.2620890799586521No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG10350.26158360439460454No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG10270.2595617021384144No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG10110.25551789762603405No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9720.24566112412710686No Hit
CACAAAGCAAAACACATCAAGGCCTGGTGCC8330.2105305724258025No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8240.20825593238758855No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG7920.20016832336282783No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7630.19283892768413843No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7570.1913225009919958No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7530.1903115498639007No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7490.18930059873580563No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7450.18828964760771053No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7390.1867732209155679No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7290.18424584309533015No Hit
GAAGTATACATGAACGACCTGGGCACCGATA6980.17641097185259322No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC6570.16604872278961855No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6490.16402682053342837No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6220.1572029004187865No Hit
CCGGAATCCTATTCCGGTGAACTGGAATGCA6050.15290635812438236No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5810.14684065135581184No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC5240.1324345977804568No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4800.12131413537141082No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC4670.11802854420510177No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4640.11727033085903045No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4530.11449021525676896No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4530.11449021525676896No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4410.11145736187248367No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG4280.10817177070617465No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4150.10488617953986559No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC4110.1038752284117705No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4030.10185332615558033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)