FastQCFastQC Report
Wed 31 May 2023
EGAF00007940941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435721
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT57871.3281434679531168No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT26330.6042857700225602No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22430.5147789525866322No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18590.426649163111257No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC16010.36743696080748917No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14830.34035541091661864No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13800.31671643092713No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA13360.3066182258830766No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA11150.25589769600271733No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9970.2288161461118468No Hit
ACTCGTCTGGAAAACATCAAAACCGACCGTA9650.22147199698889886No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9650.22147199698889886No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9540.21894744572788552No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9280.21298032456549032No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8770.201275586900792No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8520.19553797039848897No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8190.18796431661544888No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7890.18107917681268518No Hit
GAACCGATCTACGCTACCATCGACGACCTGG7890.18107917681268518No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7740.17763660691130334No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7550.173276018369553No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7520.1725875043892766No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7490.17189899040900025No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7480.17166948574890814No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7430.17052196244844753No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6620.15193208498098554No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6530.14986654304015642No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6450.14803050575941945No Hit
GCTGCTTCTAGCGATGCACTGGAAGCCCTGG6260.1436699172176691No Hit
TACGCAACCGACCCGGAATACGATCGTAAGC6090.13976833799610303No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5930.13609626343462905No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5920.1358667587745369No Hit
TTCGGTCAGGCTAAAGAAAAAGCACCGTGCA5890.13517824479426055No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5860.1344897308139842No Hit
TCCCAGGAAATCACCTTCGTTTACGAGAAAA5800.13311270285343144No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5670.13012914227223385No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5540.12714558169103624No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5510.12645706771075987No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5510.12645706771075987No Hit
CAGGACCAGGCCCAGACCAAAATGGACGATA5480.12576855373048348No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5180.1188834139277198No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5070.11635886266670645No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5030.11544084402633796No Hit
TACCTGGAGACCCAGCAGAAAATCAACCACC5000.1147523300460616No Hit
TTCATCAACATGTACACCGCACACTCCCAGA4990.11452282538596946No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4980.11429332072587733No Hit
AAACCGGCAGCGTCCCTGCTGAAACGTAACC4980.11429332072587733No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4960.1138343114056931No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4860.11153926480477186No Hit
ACCAAGACTTTCGCGAAAAAACACAACCTGA4760.10924421820385063No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA4640.10649016228274516No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4630.10626065762265302No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG4560.10465412500200817No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4460.10235907840108692No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC4460.10235907840108692No Hit
CTGGCCTCTTCTGCACCGTCCACCGCGGTTG4430.10167056442081056No Hit
GCCCAGTCCGCGACCCCGTCTGTTTCTTCCT4430.10167056442081056No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4420.10144105976071843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)