FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007940955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390419
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57611.4755941693411438No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT56651.4510052021033812No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27990.7169220760260131No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA27280.6987364856730846No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC19900.5097088000327853No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18150.4648851618389474No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17280.44260141027972516No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15510.39726550193510046No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15280.3913743952010532No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13050.3342562733883341No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12730.3260599509757466No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12560.3217056546940595No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10980.28123631278190864No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10610.27175931499235434No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10260.2627945873535868No Hit
AACTCTCCAACCGCTGAAGAGTGCGGTTACT10260.2627945873535868No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10220.26177004705201334No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9500.24332832162369147No Hit
AACTCTCCAACTGTTGAAGAATGTGGTTATA8520.21822708423514225No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG7970.20413965508850745No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7970.20413965508850745No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7280.1864663348863657No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7250.1856979296601856No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7150.18313657890625198No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7080.1813436333784985No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6860.17570866171984456No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6460.1654632587041102No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6310.16162123257320982No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6210.15905988181927622No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6160.15777920644230942No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5830.14932674895432857No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC5590.14317950714488792No Hit
AATTCCCCGAACATCGAAGCGTGCGGCTATA5490.14061815639095432No Hit
CTGCAGGCGCCGAAAACTCGCTCCACCACCA5290.13549545488308715No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5110.13088502352600667No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4400.11269943317307816No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4260.10911354211757113No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4250.10885740704217776No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC4250.10885740704217776No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4240.10860127196678442No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4100.10501538091127738No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG4070.1042469756850973No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC4060.10399084060970394No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4040.10347857045891723No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT3940.10091721970498362No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA3920.10040494955419689No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC3910.10014881447880353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)