FastQCFastQC Report
Wed 31 May 2023
EGAF00007940956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007940956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400645
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC32900.8211758539355289No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT31290.7809906525727265No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG25410.6342273084651No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC24160.6030276179660298No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG23700.5915461318623719No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG23010.5743239027068852No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22700.5665863794631157No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG21770.5433738097318074No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG21290.5313931285801644No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG20520.5121741192327371No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG17220.42980693631519173No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16260.4058455740119058No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16070.4011032210560471No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14910.37214990827290995No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG14630.3651611776011182No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA11850.29577306593118596No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11360.28354278725555043No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11110.2773028491557364No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA10530.26282619276416774No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG9840.245603963608681No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA9740.24310798836875538No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9520.23761684284091902No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG8790.219396223589462No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8700.21714984587352892No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8310.207415542437819No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8220.20516916472188595No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT8100.2021739944339752No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT8090.20192439690998265No Hit
ACGAACCTGGATGCGAATGGTACGGTTATCAACTTTAACCAGAT7460.18619975289845125No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG6870.17147349898289008No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6840.17072470641091242No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6430.16049120792721736No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG6360.15874402525926942No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5890.14701294163161902No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5600.13977461343583472No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5600.13977461343583472No Hit
GTGCTGGGAACGCGGGGTCCAAGGGCCAGCGTAGCCCGGATAGC5570.13902582086385704No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5560.13877622333986447No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5430.13553145552796117No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5260.1312882976200876No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5220.13028990752411737No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5160.128792322380162No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5130.1280435298081843No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5130.1280435298081843No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT4980.12429956694829586No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG4850.12105479913639255No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT4830.12055560408840743No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG4810.12005640904042232No Hit
ATCCGGGTGGTTACGGATTGCTTCCAGAGTTTCATCGTCGAAAT4650.11606284865654133No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4570.11406606846460082No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA4330.10807572788877934No Hit
CGGTGCCTGTGGCGGAGGCGGTTCCTGATAGCCCTGGTATGGTG4220.10533015512486116No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA4090.10208538731295785No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT4070.10158619226497273No Hit
TTCTACAACCAGTTTGAAGGATTTGTCGCCGGCAGAACCAGACA4030.10058780216900248No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4020.1003382046450099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)